Commonly used laboratory consumables for cell experiments
Tiempo de liberación:
2023-09-28
Cell culture is one of the commonly used experimental techniques in biological research, and cell culture consumables are necessary equipment and materials for cell culture experiments. Below I will summarize the experimental consumables commonly used in cell experiments-laboratory instruments and reagents for your reference:
1. Medium and culture medium: including DMEM, RPMI 1640, MEM and other different types of medium, as well as the culture medium added with fetal bovine serum, human serum, antibiotics and growth factors.
2. Centrifuge tube and centrifuge: used to collect and centrifuge cells, including 15ml and 50ml centrifuge tubes, and high speed centrifuge.
3. Pipettes and tips: used for pipetting and distributing culture medium and cell suspension, including 1ml, 5ml, 10ml pipettes and tips of corresponding specifications.
4. Microscope and cell counter: used to observe cell morphology and growth status, and cell count. Inverted microscopes and cell counters are common.
5. 96-well plate: used for high-throughput experiments, can process multiple samples at the same time, commonly used in cell proliferation, cytotoxicity and other experiments.
6. 24-well plates and 6-well plates: for larger numbers of cell culture and cell virus amplification.
7. Cell freezing tube and liquid nitrogen tank: used to preserve frozen cells.
8. Cell tubes and tips: used to observe and count cells, including acid and alkali resistant cell tubes and tips of corresponding specifications.
9. Cell aggregation transfer device: used for cell aggregation transfer and construction of three-dimensional cell model.
10. Cell Culture Cover Sheet: Used to sprinkle cell suspension on cover sheet for related experiments.
11. Aseptic operation table and sterile consumables: used for aseptic operation and aseptic operation in culture experiment, such as sterile gloves, sterile tip, etc.
12. Coating Reagents: Used to coat the surface of Petri dishes or multi-well plates to promote cell attachment and growth.
13. UV sterilizer: used for ultraviolet disinfection of workbench, culture vessel and culture tools.
14. Petri dish film applicator: Used to apply a film in a Petri dish to reduce cell attachment.
15. Cell cryopreservation solution and freezing container: used for cell cryopreservation and storage.
16. Cell lysate: used to lyse cells and extract fine
17. Cell culture shaker and incubator: used to provide temperature, humidity and CO2 control conditions to promote cell growth and proliferation.
18. Vacuum pump and needle filter: used to prepare sterile culture medium and filter out particles and microorganisms in cell culture solution.
19. Homogenizer and centrifugal screen: used to homogenize cell suspension to obtain single cell suspension.
20. Cell culture incubator and CO2 controller: provide constant temperature and CO2 concentration to maintain the ideal growth environment for cells.
21. Centrifuge tube rack and centrifuge tube rotor: used to install centrifuge tubes and perform high-speed centrifugation to separate cells and culture media.
22. Culture Vessel Scraper and Cell Dissociation Solution: Used to strip and collect cells from culture vessels.
23. PCR instrument and RT-PCR kit: used for gene expression analysis and cell molecular biology experiments.
24. Microscope slides and stains: used for cell morphology observation and staining experiments.
25. Real-time cell analyzer: for real-time monitoring of cell proliferation, migration and invasion.
26. Polymeric vectors and transfection reagents: for gene transfection and protein expression.
27. Cell Cracker and Extracts: Used to lyse cells and extract proteins or nucleic acids.
28. Protein assay kit and photometer: used to determine the protein content in cell culture.
29. Cell proliferation reagent: such as MTT reagent, CCK-8 reagent, etc., used for measuring cell proliferation ability.
30. Apoptosis detection kit: used to detect the degree of apoptosis.
31. Low temperature refrigerator, -80 refrigerator and liquid nitrogen tank: mainly used for the preservation of reagents and cells
The above are the commonly used experimental consumables in cell culture-laboratory instruments and reagents. Different consumables may be used for different experiments and requirements. Choosing the right cell culture consumables can improve the experimental results and data reliability. At the same time, it is also necessary to choose the appropriate brand and quality assurance according to the experimental purpose and requirements.
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